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Saturación del Mapa Genético de una población Intra-Acervo de Fríjol (Phaseolus Vulgaris L.) empleando Marcadores Moleculares tipo SSR y SSCP
dc.contributor.advisor | Blair, Matthew | |
dc.contributor.author | Franco Herrera, Natalia | |
dc.date.accessioned | 2024-04-05T16:55:18Z | |
dc.date.available | 2024-04-05T16:55:18Z | |
dc.date.issued | 2011-07 | |
dc.identifier.uri | https://bdigital.uniquindio.edu.co/handle/001/6768 | |
dc.description.abstract | El mapa obtenido presentó una longitud total de 2439,7cM, con una distancia promedio entre marcadores de 4,9cM. El GL más largo fue el B02 con 342,6cM, mientras que el B05 fue el más corto con 143,3cM. Para incrementar el número de marcadores, se diseñaron marcadores basados en genes de nodulación, flanqueando regiones intrónicas. En total se lograron diseñar 204 marcadores, pero solamente se lograron mapear 12 (dos en DB y 10 en DG). Entre los marcadores anclados al mapa DG, se encontraron los marcadores basados en los genes NIN y LHK1. El primero es un regulador en la etapa de infección controla la curvación de los pelos de la raíz; el otro es un receptor quinasa de citoquinina (implicado en la organogénesis de nódulos de la corteza). Respecto a la búsqueda de SNPs, se lograron reportar 343, de los cuales 150 fueron localizados entre los genotipos DOR364 y BAT477 y 193 entre DOR364 y G19833. En siete de las 44 secuencias analizadas no se encontraron SNPs. Entre los genotipos DOR364 y BAT477 las mutaciones tipo transversión fueron más abundantes (67,4%) que las transiciones (32,6%) y para DOR364 y G19833, ambos tipos de mutaciones se presentaron casi en iguales proporciones (50,3% de transiciones y 49,7% de transversiones). | spa |
dc.description.tableofcontents | 1.Introducción 14-- 2. Materiales y métodos 41-- 3. Resultados 49-- 4. Discusión 49--5. Conclusiones 75-- 6. Bibliografía --77 | spa |
dc.format.extent | 122 páginas | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.publisher | Universidad del Quindío Centro Internacional de Agricultura Tropical (CIAT) | spa |
dc.rights | Derechos reservados Universidad del Quindío | eng |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | spa |
dc.title | Saturación del Mapa Genético de una población Intra-Acervo de Fríjol (Phaseolus Vulgaris L.) empleando Marcadores Moleculares tipo SSR y SSCP | spa |
dc.type | Trabajo de grado - Pregrado | spa |
dcterms.audience | Estudiantes y Docentes Universidad del Quindío | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) | spa |
dc.subject.proposal | Phaseolus vulgaris L. | spa |
dc.subject.proposal | Mapeo genético | spa |
dc.subject.proposal | SSR | spa |
dc.subject.proposal | SSCP | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.version | info:eu-repo/semantics/draft | spa |
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dc.contributor.corporatename | Universidad del Quindío | spa |
dc.description.degreelevel | Pregrado | spa |
dc.description.degreename | Biólogo | spa |
dc.identifier.instname | Universidad del Quindio | spa |
dc.identifier.reponame | Repositorio Institucional | spa |
dc.identifier.repourl | https://bdigital.uniquindio.edu.co | spa |
dc.publisher.faculty | Facultad de Ciencias Básicas y Tecnologías | spa |
dc.publisher.place | Armenia-Quindío | spa |
dc.publisher.program | Ciencias Básicas y Tecnologías - Biología | spa |
dc.type.content | Text | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TP | spa |
dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_14cb | spa |